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Cgastm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Cgastm1a(EUCOMM)Hmgu
Name: cyclic GMP-AMP synthase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4435548
Synonyms: cGAS-, Mb21d1tm1a(EUCOMM)Hmgu
Gene: Cgas  Location: Chr9:78430526-78443237 bp, - strand  Genetic Position: Chr9, 43.65 cM
Mutation
origin
Mutant Cell Lines:  HEPD0516_3_C04, HEPD0516_3_D04, HEPD0516_3_E03, HEPD0516_3_G04, HEPD0516_3_H04
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 78437955 of Chromosome 9 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78437075. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Cgas Mutation:  14 strains or lines available
Notes
Cell line HEPD0516_3_C04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:201167
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  22 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory