Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Srftm1Nor
Name:	serum response factor; targeted mutation 1, Alfred Nordheim
MGI ID:	MGI:2158696
Gene:	Srf  Location: Chr17:46859255-46867101 bp, - strand  Genetic Position: Chr17, 22.9 cM
Alliance:	Srftm1Nor page

Germline Transmission:	Earliest citation of germline transmission: J:50876
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: An 880 bp genomic fragment containing the initiation codon and sequences encoding the dimerization and DNA binding domains was replaced with a neomycin selection cassette. RT-PCR analysis showed that no detectable transcript was produced from this allele in mutant embryos (data not shown). Activity assays in extracts derived from homozygous E8.5 embryos demonstrated that no functional protein was present, and immunohistochemistry experiments on sections of E8.5 homozygous embryos confirmed that no encoded protein was detectable. (J:50876)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Srf Mutation:	25 strains or lines available

Original:	J:50876 Arsenian S, et al., Serum response factor is essential for mesoderm formation during mouse embryogenesis. EMBO J. 1998 Nov 2;17(21):6289-99
All:	4 reference(s)