Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tcrbtm1(Tcrb)Fwa
Name:	T cell receptor beta chain; targeted mutation 1, Frederick W Alt
MGI ID:	MGI:3775389
Synonyms:	Vbeta14Rep
Gene:	Tcrb  Location: Chr6:40868230-41535305 bp, + strand  Genetic Position: Chr6, cytoband A-C

Germline Transmission:	Earliest citation of germline transmission: J:131995
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Inserted expressed sequence) Mutations:    Insertion, Intergenic deletion   Mutation details: ES cells containing the Tcrbtm1Slek mutation that lacks the Dbeta2-Jbeta2 locus were again targeted to replace the Vbeta14 recombination signaling sequence (RSS) with germline Dbeta1 and Jbeta1.1 segments, their RSS, and intervening intronic sequence. A floxed neo resistance cassette used in the targeting was subsequently removed by transient Cre expression. The gene targeting also introduced a loxP site and an EcoRI site to distinguish between the targeted allele and the endogenous Vbeta 14 allele. Southern blots of thymus confirmed the presence of both germline and recombined versions of the knocked in gene segments. (J:131995)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tcrb Mutation:	94 strains or lines available

Original:	J:131995 Ranganath S, et al., Productive coupling of accessible Vbeta14 segments and DJbeta complexes determines the frequency of Vbeta14 rearrangement. J Immunol. 2008 Feb 15;180(4):2339-46
All:	1 reference(s)