Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Prkacatm3Gsm
Name:	protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 3, G Stanley McKnight
MGI ID:	MGI:3610390
Synonyms:	CalphaR
Gene:	Prkaca  Location: Chr8:84699622-84723072 bp, + strand  Genetic Position: Chr8, 40.22 cM, cytoband C3
Alliance:	Prkacatm3Gsm page

Germline Transmission:	Earliest citation of germline transmission: J:103418
Parent Cell Line:	REK2 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion   Mutation details: A W196R mutation was introduced into exon 7 by homolgous recombination. A loxP flanked neomycin resistance gene containing the SV40 polyA sequence was inserted into intron 5. Western blot demonstrated that mutant cells express half of the level of protein as that of wild-type cells. Cre-mediated recombination restores the expression of the mutant transcript containing the point mutation, and expression of the mutant protein. (J:103418)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prkaca Mutation:	22 strains or lines available

Original:	J:103418 Niswender CM, et al., Cre recombinase-dependent expression of a constitutively active mutant allele of the catalytic subunit of protein kinase A. Genesis. 2005 Nov;43(3):109-19
All:	8 reference(s)