Prkntm1Tmd
Targeted Allele Detail
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Symbol: |
Prkntm1Tmd |
Name: |
parkin RBR E3 ubiquitin protein ligase; targeted mutation 1, Ted M Dawson |
MGI ID: |
MGI:3050230 |
Gene: |
Prkn Location: Chr17:11059271-12282248 bp, + strand Genetic Position: Chr17, 7.8 cM, cytoband A3.2-A3.3
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Alliance: |
Prkntm1Tmd page
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Decrease in tyrosine hydroxylase (TH) containing neurons in the locus coeruleus (LC) of Prkntm1Tmd/Prkntm1Tmd mice
Show the 1 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:91487
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
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Mutation details: Cre-mediated recombination excised exon 7 and a 3' FRT-flanked neo, which had been inserted via homologous recombination, in the germ line. Because exon 7 contains a nonintegral number of codons, a deletion of it results in a frame shift after amino acid 243 and premature stop codon in exon 8 after 8 amino acids. Northern blot detected transcript slightly smaller than that of wild-type, and RT-PCR confirmed it was the result of the deletion of exon 7. Western blot failed to detect protein in mutant mice brains.
(J:91487)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Prkn Mutation: |
54 strains or lines available
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Original: |
J:91487 Von Coelln R, et al., Loss of locus coeruleus neurons and reduced startle in parkin null mice. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10744-9 |
All: |
12 reference(s) |
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