Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	En2tm3.1Alj
Name:	engrailed 2; targeted mutation 3.1, Alexandra L Joyner
MGI ID:	MGI:1930812
Synonyms:	En2pbd
Gene:	En2  Location: Chr5:28370692-28377164 bp, + strand  Genetic Position: Chr5, 13.94 cM
Alliance:	En2tm3.1Alj page

Germline Transmission:	Earliest citation of germline transmission: J:60433
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Replacement of two adjacent Pax-binding sites (66 bp) in the regulatory region with a lox-P flanked neomycin cassette. In this derivative of En2tm3Alj, the neomycin cassette was removed via Cre mediated recombination, leaving a loxP site in place of the Pax binding sites. (J:60433) Inheritance:    Recessive

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

11 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any En2 Mutation:	104 strains or lines available

Original:	J:60433 Li Song D, et al., Two Pax2/5/8-binding sites in Engrailed2 are required for proper initiation of endogenous mid-hindbrain expression. Mech Dev. 2000 Feb;90(2):155-65
All:	1 reference(s)