Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Ighmtm1Msn
Name:	immunoglobulin heavy constant mu; targeted mutation 1, Michael S Neuberger
MGI ID:	MGI:2447178
Synonyms:	mus-, sIgM-, Smu-
Gene:	Ighm  Location: Chr12:113382446-113386350 bp, - strand  Genetic Position: Chr12, 62.1 cM, cytoband F1-2
Alliance:	Ighmtm1Msn page

Germline Transmission:	Earliest citation of germline transmission: J:49405
Parent Cell Line:	CCB (ES Cell)
Strain of Origin:	129S/SvEv-Gpi1c

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A secretory isoform specific mutation was achieved by deleting a 1.8 kb fragment encompassing sequence encoding a portion of the secretory tailpiece and the stop codon and polyadenylation sites. A single loxP site was left in place of the deleted sequence after the in vitro removal of the floxed tk-neo selection. Serum Igh-6 was undetectable in homozygous mutant mice. Flow cytometric analysis showed increased expression of the surface isoform. Study of heterozygous mice using allotype specific antibodies indicated that the augmented surface expression was a result of affected RNA processing rather than compensation. (J:49405)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ighm Mutation:	55 strains or lines available

Original:	J:49405 Ehrenstein MR, et al., Targeted gene disruption reveals a role for natural secretory IgM in the maturation of the primary immune response. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10089-93
All:	16 reference(s)