Analysis Tools
homeostasis/metabolism
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• pancreatic islets isolated from 12-week-old mice show a significant decrease in intracellular insulin content
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• mice are hyperglycemic with significantly increased ad libitum blood glucose levels at all time points between P0 and 12 months of age
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• mice are glucose intolerant at 6 weeks of age
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endocrine/exocrine glands
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• immunofluorescent staining of islet hormones shows a small number of bihormonal cells co-expressing insulin and somatostatin (INS+/SST+) at 6 weeks of age
• however, no visible defects in delamination of the endocrine population are noted at P0
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• pancreatic islets isolated from 6-week-old mice show an obvious decrease in the number of INS+ beta cells with no apparent defect in the other endocrine cell types
• however, E18.5 islets are largely normal with only a trend towards decreased beta cell numbers and no defects in the number of non-beta endocrine cells or levels of the other endocrine hormones
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• at E18.5, pancreata show a small, but significant, decrease in Ins1 (insulin I), Ins2 (insulin II) and Gcg (glucagon) mRNA expression
• islets isolated from 8-week-old mice show a 3-fold increase in mRNA expression of Tle4 (Grg4), but no change in Tle1 (Grg1), suggesting that Tle4 upregulation is compensating for the loss of Tle3 (Grg3) and preventing a more severe pancreas phenotype
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• pancreatic islets isolated from 12-week-old mice show a significant decrease in intracellular insulin content
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growth/size/body
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• mice exhibit normal body weight at 4-6 weeks of age
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