Phenotypes for Rad51 Tg(Ddx4-cre)1Dcas MGI:7311694 MGI Mouse

decreased male germ cell number ( J:324159 )

• at P12 and P60, Hematoxylin staining of seminiferous tubules showed complete germ cell loss

• at P10, no PLZF+ early spermatogonial stem cells (SSCs) are detected in seminiferous tubules

• however, GCNA+ male germ cells at still present at E18.5

absent spermatogonia ( J:324159 )

• at P8, TEM showed complete loss of spermatogonia in seminiferous tubules

• at P8, testes show severe depletion of germ cells in the seminiferous tubules; only SOX9+ Sertoli cells are detected at P8

increased Sertoli cell number ( J:324159 )

• at P19 and P40, the number of SOX9+ Sertoli cells per tubule is significantly higher than in control testes

small testis ( J:324159 )

• at P40, testis size is significantly smaller than in control males

decreased testis weight ( J:324159 )

• from P10 to P40, testis weight is significantly lower than in control males

• however, testis weight is normal at P8

abnormal spermatogenesis ( J:324159 )

• at P12, seminiferous tubules display complete loss of spermatogonia and a Sertoli cell-only phenotype

• immunofluorescence staining of the Sertoli cell marker SOX9 showed that tubules only contain Sertoli cells at P8

abnormal spermatocyte morphology ( J:324159 )

• at P12, seminiferous tubules show complete loss of leptotene and zygotene spermatocytes

abnormal male meiosis ( J:324159 )

• immunofluorescence staining of meiotic markers showed no SYCP3+ or gammaH2AX+ cells in seminiferous tubules at P8

male infertility ( J:324159 )

• adult male mice mated with wild-type fertile females for at least 6 months produce no pups