Phenotypes Associated with This Genotype

Genotype
MGI:6887744

• Allelic Composition: Gt(ROSA)26Sor^{tm1(cre/ERT2)Thl}/Gt(ROSA)26Sor⁺; Pknox1^tm1.1Rygo/Pknox1^tm1.1Rygo
• Genetic Background: involves: 129 * C57BL/6 * C57BL/6JJcl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:258387 )

♂N • three weeks after tamoxifen treatment, adult testis size and weight is markedly but not significantly decreased relative to similarly treated controls

abnormal spermatogonia morphology ( J:258387 )

♂ • after tamoxifen treatment, GFRalpha1+ cells (corresponding to A_{single~paired} spermatogonia in control testis) form A_aligned-like morphologies in adult testes

♂ • c-Kit+ cells aligned more than 32 appear to be absent whereas A_aligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ A_aligned spermatogonia fail to differentiate into A1 spermatogonia

decreased male germ cell number ( J:258387 )

♂ • after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells

azoospermia ( J:258387 )

♂ • epididymides of tamoxifen-treated adult males are devoid of sperm

increased testis apoptosis ( J:258387 )

♂ • after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls

seminiferous tubule degeneration ( J:258387 )

♂ • after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist

♂ • most seminiferous tubules lack SCP3+ meiotic cells

arrest of spermatogenesis ( J:258387 )

♂ • after tamoxifen treatment, adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular

abnormal spermatogonia morphology ( J:258387 )

♂ • after tamoxifen treatment, GFRalpha1+ cells (corresponding to A_{single~paired} spermatogonia in control testis) form A_aligned-like morphologies in adult testes

♂ • c-Kit+ cells aligned more than 32 appear to be absent whereas A_aligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ A_aligned spermatogonia fail to differentiate into A1 spermatogonia

decreased male germ cell number ( J:258387 )

♂ • after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells

azoospermia ( J:258387 )

♂ • epididymides of tamoxifen-treated adult males are devoid of sperm

increased testis apoptosis ( J:258387 )

♂ • after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls

endocrine/exocrine glands

increased testis apoptosis ( J:258387 )

♂ • after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls

seminiferous tubule degeneration ( J:258387 )

♂ • after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist

♂ • most seminiferous tubules lack SCP3+ meiotic cells