Phenotypes Associated with This Genotype

Genotype
MGI:6505536

• Allelic Composition: Med19^{tm1c(EUCOMM)Wtsi}/Med19^{tm1c(EUCOMM)Wtsi}; Tg(Adipoq-icre/ERT2)1Soff/0
• Genetic Background: involves: C57BL/6J * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

growth/size/body region phenotype ( J:298822 )

N • mice show no significant change in body weight at 21 days after a 5-day tamoxifen treatment initiated at 12 weeks of age

adipose tissue

whitened brown adipose tissue morphology ( J:298822 )

• brown adipose tissue (BAT) accumulates unilocular lipid droplets, indicating whitening of brown fat

• Oil red O staining confirmed that pockets of white-adipocyte-like cells within BAT are adipocytes

decreased white fat cell number ( J:298822 )

• marked reduction of white adipocytes at 21 days after tamoxifen treatment

abnormal white fat cell size ( J:298822 )

• irregularly sized white adipocytes in residual white fat tissue at 21 days after tamoxifen treatment

decreased gonadal fat pad weight ( J:298822 )

• dramatic reduction in gonadal WAT tissue (gWAT) mass at 21 days after tamoxifen treatment

decreased inguinal fat pad weight ( J:298822 )

• dramatic reduction in inguinal WAT tissue (iWAT) mass at 21 days after tamoxifen treatment

decreased white adipose tissue mass ( J:298822 )

• dramatic reduction in white adipose tissue mass at 21 days after tamoxifen treatment

lipodystrophy ( J:298822 )

• loss of WAT due to increased apoptosis and macrophage infiltration

increased white adipose tissue apoptosis ( J:298822 )

• accumulation of apoptotic cells in iWAT after tamoxifen treatment, as determined by cleaved caspace-3 staining

white adipose tissue inflammation ( J:298822 )

• increased white adipose tissue (WAT) inflammation with significant macrophage infiltration in gWAT and iWAT after tamoxifen treatment

homeostasis/metabolism

impaired lipolysis ( J:298822 )

• surprisingly, lipolytic activity is impaired in inguinal WAT tissue (iWAT) explants, as shown in a lipolysis assay

• mRNA expression of lipolysis genes is reduced in iWAT

immune system

white adipose tissue inflammation ( J:298822 )

• increased white adipose tissue (WAT) inflammation with significant macrophage infiltration in gWAT and iWAT after tamoxifen treatment

cellular

increased white adipose tissue apoptosis ( J:298822 )

• accumulation of apoptotic cells in iWAT after tamoxifen treatment, as determined by cleaved caspace-3 staining