Phenotypes Associated with This Genotype

Genotype
MGI:6430478

• Allelic Composition: Ifng^tm1Yiw/Ifng^tm1Yiw
• Genetic Background: B6.129X1-Ifng^tm1Yiw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

peritoneal inflammation ( J:50405 )

• JHMV infected mice develop peritonitis at 21 dpi

• JHMV infection is characterized by disseminated granulomatous inflammation and exudative fibrinous serositis with plasma cells and eosinophils in the abdominal cavity and these same lesions in the thoracic cavity

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the C57BL/6 background as on BALB/c background

liver inflammation ( J:50405 )

• mice injected with an anti-CD8 monoclonal antibody to deplete CD8+ T cells and then infected with JHMV develop severe hepatitis and die within 2 weeks

• however, JHMV infected mice without depletion of CD8+ T cells do not develop hepatitis

increased susceptibility to Coronaviridae infection ( J:50405 , J:91691 )

• mice show increased susceptibility to infection with murine coronavirus strain JHM (hepatitis mouse virus) , showing higher viral titers at 3 and 5 dpi with a gradual decrease but persistence of virus in the liver compared to viral clearing in the liver of controls, higher antibody titers and virus neutralization antibody tiers (J:50405)

• virus is recovered from various tissues, including the liver, spleen, kidney, pancreas, mesenterium and lung of some JHMV-infected mice at 21 dpi, with the highest level of viral growth in the mesenterium (J:50405)

• although both cell-mediated and humoral immune responses are activated during JHMV infection, JHMV persists in mice (J:50405)

• Background Sensitivity: viral titer of JHMV infected mice is slightly higher than in heterozygotes on a C57BL/6 background compared to a much higher viral titer in mice on a BALB/c background (J:91691)

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:50405 , J:91691 )

• JHMV infected mice show some lethality at 16 days post infection (dpi), with survival rate gradually decreasing such that about 90% die by 50 dpi (J:50405)

• JHMV-infected mice treated with recombinant IFN-gamma show increases survival time (J:50405)

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) show a gradual decrease in survival rate on the C57BL/6 background compared to lethality within 1 week on a BALB/c background (J:91691)

cellular

focal hepatic necrosis ( J:91691 )

• Background Sensitivity: JHMV infected mice show only focal necrotic lesion in the liver at 5 days post infection (dpi) on a C57BL/6 background compared to massive liver necrosis on a BALB/c background

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:50405 , J:91691 )

• JHMV infected mice show some lethality at 16 days post infection (dpi), with survival rate gradually decreasing such that about 90% die by 50 dpi (J:50405)

• JHMV-infected mice treated with recombinant IFN-gamma show increases survival time (J:50405)

• Background Sensitivity: mice infected with the DL variant of the coronavirus mouse hepatitis virus (JHMV) show a gradual decrease in survival rate on the C57BL/6 background compared to lethality within 1 week on a BALB/c background (J:91691)

digestive/alimentary system

peritoneal inflammation ( J:50405 )

• JHMV infected mice develop peritonitis at 21 dpi

• JHMV infection is characterized by disseminated granulomatous inflammation and exudative fibrinous serositis with plasma cells and eosinophils in the abdominal cavity and these same lesions in the thoracic cavity

liver/biliary system

liver inflammation ( J:50405 )

• mice injected with an anti-CD8 monoclonal antibody to deplete CD8+ T cells and then infected with JHMV develop severe hepatitis and die within 2 weeks

• however, JHMV infected mice without depletion of CD8+ T cells do not develop hepatitis

abnormal liver morphology ( J:50405 )

• JHMV infected mice show larger liver lesions that are infiltrated with neutrophils compared to wild-type infected mice

• some JHMV infected mice exhibit pseudomembrane on the surface of the liver at 14 dpi

• however, JHMV-infected mice do not develop severe hepatitis and alanine transaminase levels are similar to those in infected wild-type mice

focal hepatic necrosis ( J:91691 )

• Background Sensitivity: JHMV infected mice show only focal necrotic lesion in the liver at 5 days post infection (dpi) on a C57BL/6 background compared to massive liver necrosis on a BALB/c background

homeostasis/metabolism

abnormal circulating interferon level ( J:91691 )

• JHMV infected mice show increased levels of IFN activity; similar level of IFN activity is seen on the C57BL/6 background as on BALB/c background

ascites ( J:50405 )

• about 50% of JHMV infected mice exhibit accumulation of ascites fluid at P21

• however, no bacteria is isolated from ascites fluid

growth/size/body

abnormal abdominal wall morphology ( J:50405 )

• some JHMV infected mice exhibit organs in abdominal cavities that adhere to each other and the peritoneum

abnormal thoracic cavity morphology ( J:50405 )

• JHMV infected mice show accumulatio of a translucent fluid in the thoracic cavity in the subacute phase

behavior/neurological

abnormal behavior ( J:50405 )

• a small number of JHMV infected mice show signs of central nervous system disease such as inability to turn over rapidly