Phenotypes Associated with This Genotype

Genotype
MGI:6408698

• Allelic Composition: Taf12^em1(IMPC)Mbp/Taf12^em1(IMPC)Mbp
• Genetic Background: C57BL/6NCrl-Taf12^em1(IMPC)Mbp/Mmucd

Data Sources

IMPC Data for Taf12

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Taf12^em1(IMPC)Mbp/Taf12^em1(IMPC)Mbp mice exhibit embryonic lethality, with embryos recovered at E3.5 as blastocysts but not at E7.5. Blastocysts in vitro hatch from the zona pellucida but outgrowths lack an obvious inner cell mass colony.

embryo

embryonic growth arrest ( J:348275 )

• embryos are arrested before progressing to an egg-cylinder

absent inner cell mass ( J:279207 , J:348275 )

• in vitro outgrowths lack an obvious ICM colony after 3 days in culture (J:279207)

• blastocysts fail to form a conspicuous ICM colony after 3 days in culture, suggesting loss of pluripotent cells during in vitro outgrowth (J:348275)

• RNA seq on in vitro outgrowths (E3.5 + 72 h in culture) showed alterations in expression of many pluripotency-associated genes (J:348275)

• however, trophoblast giant cells, derived from the trophectoderm, appear phenotypically normal and NANOG+ and SOX17+ cell populations and specificity are normal (J:348275)

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - UCD

preweaning lethality, complete penetrance ( J:211773 )

IMPC - UCD

embryonic lethality prior to organogenesis, complete penetrance ( J:279207 )

embryonic lethality at implantation, complete penetrance ( J:348275 )

• embryos exhibit peri-implantation lethality; embryos are recovered at Mendelian ratios and appear as morphologically normal blastocysts at E3.5 but are not present at E7.5

reproductive system

abnormal embryo implantation ( J:279207 )

• in vitro, blastocysts do hatch from the zona pellucida but form irregular outgrowths with no obvious ICM colony but normal NANOG+ and SOX17+ cells after 3 days in culture

empty decidua capsularis ( J:348275 )

• 14 of 45 (31%) decidua from heterozygous crosses are empty at E6.5 and E7.5

failure of embryo implantation ( J:348275 )

• embryos exhibit a failure to implant post-blastocyst formation