Phenotypes for Patl2 MGI:6200082 MGI Mouse

reproductive system phenotype ( J:264835 )

N	• male fertility is normal

increased primordial ovarian follicle number ( J:264835 )

• increase in the number of primordial oocytes at P26 but not at P12 compared to wild-type controls

• numbers of primary and secondary follicles are similar at P12 and P26

abnormal meiotic spindle morphology ( J:264835 )

• significant increase in the number of MII oocytes with abnormal spindle morphology, misalignment of chromosomes on the spindle and numerous cytoplasmic asters

abnormal oogenesis ( J:264835 )

• oocyte growth is impaired

• increase in the percentage of oocytes released at stages before MII, 45% compared to 26% in wild-type controls

abnormal oocyte morphology ( J:264835 )

• following pregnant mare serum gonadotropin ovarian stimulation, germinal vesicle oocytes are smaller in diameter compared to similarly treated wild-type controls

• however, the number of oocytes produced by stimulation is similar to controls

• unlike human patients, stimulated mice produce MII stage oocytes but these are also smaller in diameter compared to controls

abnormal female meiosis ( J:264835 )

• oocyte meiotic maturation is impaired

• significant increase in the number of MII oocytes with abnormal spindle morphology, misalignment of chromosomes on the spindle and numerous cytoplasmic asters

abnormal female meiosis I arrest ( J:264835 )

• oocytes apparently arrested at MI display misaligned chromosomes and abundant cytoplasmic asters

reduced female fertility ( J:264835 )

• severe reduction with both total number of pups and litters per month reduced over a 6 month period when crossed to wild-type males

• fertilized zygotes show numerous defects, including delayed pronucleus formation, absence of sperm DNA decondensation, and/or polyspermy

• only 27.2% +/- 5.1 of 2-cell embryos reach the blastocyst stage compared to 87.1% +/- 5.6% for wild-type controls

decreased litter size ( J:264835 )

• average litter size is 2.3 +/- 0.4 compared to 7.3 +/- 0.8 for wild-type controls when both are crossed to wild-type males

polyspermy ( J:264835 )

• fertilized zygotes show numerous defects, including polyspermy

impaired fertilization ( J:264835 )

• decrease in the percentage of eggs reaching the 2-cell stage following IVF to 36.4% +/- 6.4%, estimated to be a 65% success rate for MII stage oocytes compared to 90% for wild-type controls