Patl2^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Patl2^{tm1b(EUCOMM)Hmgu}
• Name: protein associated with topoisomerase II homolog 2 (yeast); targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6200023
• Gene: Patl2 Location: Chr2:121950589-122016670 bp, - strand Genetic Position: Chr2, 60.53 cM, cytoband F1
• Alliance: Patl2^{tm1b(EUCOMM)Hmgu} page
• IMPC: Patl2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264835
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 121956938 of Chromosome 2 upstream of the exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 7 at position 121957459. Exon 7 is thus flanked by loxP sites. Cre-mediated recombination removed exon 7 creating a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtm (J:157065, J:264835)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Patl2 Mutation: 32 strains or lines available

References

• Original: J:264835 Christou-Kent M, et al., PATL2 is a key actor of oocyte maturation whose invalidation causes infertility in women and mice. EMBO Mol Med. 2018 May;10(5)
• All: 2 reference(s)