Phenotypes Associated with This Genotype

Genotype
MGI:5762596

• Allelic Composition: Csnk1a1^{tm1c(KOMP)Wtsi}/Csnk1a1^{tm1c(KOMP)Wtsi}; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * CBA
• Cell Lines: EPD0270_3_B02

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:216977 )

• mice die 5-17 days after induction with polyinosinic-polycytidylic acid [poly(I:C)]

cardiovascular system

abnormal myocardium layer morphology ( J:216977 )

• areas of the myocardium show wavy fibers with loss of transversal striations, partially with loss of the cell nucleus following poly(I:C) induction

cardiac ischemia ( J:216977 )

• myocardium shows signs of ischemia following poly(I:C) induction

hematopoietic system

abnormal blood cell morphology ( J:216977 )

• mice exhibit a decrease in all peripheral blood counts after induction with poly(I:C)

extramedullary hematopoiesis ( J:216977 )

• seen following poly(I:C) induction

decreased hematopoietic stem cell number ( J:216977 )

• 10 days after poly(I:C) treatment, mice show a reduction in hematopoietic stem cells (LSK; Lin^lowSca-1+ckit+), including long-term (LT; Lin^lowSca-1+ckit+CD150+CD48-) and short-term (ST; Lin^lowSca-1+ckit+CD150-CD48-) hematopoietic stem cells and multipotent progenitor cells (MPPs; Lin^lowSca-1+ckit+CD150-CD48+)

abnormal spleen red pulp morphology ( J:216977 )

• rarefication of the red pulp is seen following poly(I:C) induction

abnormal hematopoietic stem cell physiology ( J:216977 )

• a greater number of LSK cells are undergoing early and late apoptosis after induction with poly(I:C) compared to controls

• hematopoietic stem cells (HSC) from mice induced with poly(I:C) exit quiescence and enter the cell cycle, with a decrease in the number of HSC (LSK) in G0 and an increase in S/G2/M compared to control cells

bone marrow failure ( J:216977 )

• mice show evidence of fulminant bone marrow failure with evidence of ischemia in multiple organs after induction with poly(I:C)

• lethally irradiated wild-type mice transplanted with mutant whole bone marrow cells become moribund with bone marrow failure 8-14 days after poly(I:C) treatment, with a complete loss of HSPCs

immune system

abnormal spleen red pulp morphology ( J:216977 )

• rarefication of the red pulp is seen following poly(I:C) induction

bone marrow failure ( J:216977 )

• mice show evidence of fulminant bone marrow failure with evidence of ischemia in multiple organs after induction with poly(I:C)

• lethally irradiated wild-type mice transplanted with mutant whole bone marrow cells become moribund with bone marrow failure 8-14 days after poly(I:C) treatment, with a complete loss of HSPCs

liver/biliary system

abnormal liver morphology ( J:216977 )

• cytoplasmic vacuolization and pyknotic nuclei are seen in the liver following poly(I:C) induction

abnormal liver physiology ( J:216977 )

• liver shows signs of ischemia following poly(I:C) induction

muscle

abnormal myocardium layer morphology ( J:216977 )

• areas of the myocardium show wavy fibers with loss of transversal striations, partially with loss of the cell nucleus following poly(I:C) induction