Phenotypes Associated with This Genotype

Genotype
MGI:5754963

• Allelic Composition: Nlgn1^tm1.1Sud/Nlgn1^tm1.1Sud; Nlgn2^tm1.1Sud/Nlgn2^tm1.1Sud; Nlgn3^tm4.1Sud/Y; Tg(Pcp2-cre)2Mpin/?
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal synaptic bouton morphology ( J:227671 )

• bouton size is reduced

abnormal synapse morphology ( J:227671 )

• 50% decrease in density of climbing fiber synapse on distal Purkinje cell dendrites

• 15% decrease in density of climbing fiber synapse on proximal Purkinje cell dendrites

• inhibitory basket/stellate cell synapses are increased in size, but not number

• 25% decrease in size of climbing fiber synapse on distal and proximal Purkinje cell dendrites

• increase in puncta density as detected by GAD65

• parallel fiber synapse density is not altered

abnormal CNS synaptic transmission ( J:227671 )

• decreased frequency of delayed quantal release events, but not amplitude events

• impaired synaptic transmission at GABAergic synapses

decreased excitatory postsynaptic current amplitude ( J:227671 )

• 40% decrease in amplitude of climbing fiber EPSCs in juvenile and adult mice

• however, no change in kinetics or paired pulse ratio of climbing fiber EPSCs and no alterations in parallel-fiber EPSCs

abnormal inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in IPSC amplitude in Purkinje cells, but no changes in kinetics or paired pulse ratio

abnormal miniature inhibitory postsynaptic currents ( J:227671 )

• 80% decrease in mIPSC frequency and amplitude (45%) in basket/stellate cell synapses

• decrease in decay, but not rise times of mIPSCs

decreased miniature inhibitory postsynaptic current amplitude ( J:227671 )

• 45% decrease in basket/stellate cell synapses

decreased miniature inhibitory postsynaptic current frequency ( J:227671 )

• 80% decrease in basket/stellate cell synapses