Analysis Tools
hematopoietic system
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• modified IDH2 expression induced by doxycycline in chow starting around 3 weeks
• peripheral blood cell counts, number of hematopoietic stem cells, myeloid progenitors, mature B and T lymphocytes, and myeloid cells all normal when examined 8 weeks after induction
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• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal
• differentiation blockade is removed when doxycycline is removed from the culture medium
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• by 7 months KSL cells are increased in bone marrow relative to controls
• long-term hematopoietic stem cells also tend to expand in number
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• about 1.3X larger than control spleens
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• significant extramedullary hematopoiesis
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neoplasm
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• infection of KSL cells with retrovirus containing HoxA9 and Meis1 and then injected into sub-lethally irradiated mice results in a pre-leukemic cell expansion
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cellular
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• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal
• differentiation blockade is removed when doxycycline is removed from the culture medium
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• by 7 months KSL cells are increased in bone marrow relative to controls
• long-term hematopoietic stem cells also tend to expand in number
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immune system
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• about 1.3X larger than control spleens
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growth/size/body
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• about 1.3X larger than control spleens
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