Analysis Tools
mortality/aging
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• the 2 pups that survived past birth had to be euthanized by P7
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• 1 of 3 pups died at birth
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respiratory system
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• develop large, cyst-like structures at the distal epithelial branch tips that are the result of larger, rounder branch tips
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• cultured lungs from E12.5 embryos show a significant reduction in branching
• branch tips are larger and rounder and lead to large open spaces in the lung
• significant decrease in total lung epithelial cell proliferation at E14.5 resulting from a decrease in proliferation of the distal tip epithelium
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• distal epithelial tips show a range of defects including absence of microvilli, apical membranous blebs and disrupted cell-cell adhesion
• the basal epithelial surface is not uniform and many cells have a rounded appearance with membranous blebs projecting into the subcellular space
• the extracellular matrix and stabilized tubulin appear disrupted at the basal surfaces
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small lung
(
J:202984
)
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• noticeable by E14.5 and more significant by E16.5
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• pups that survive birth have difficulty breathing
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cellular
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• apparent precocious differentiation of distal tip progenitor cells into type 2 alveolar cells at E14.5
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• migration of epithelial cells from cultured E12.5 lung buds is decreased compared to controls
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• significant decrease in total lung epithelial cell proliferation at E14.5 resulting from a decrease in proliferation of the distal tip epithelium
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growth/size/body
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• develop large, cyst-like structures at the distal epithelial branch tips that are the result of larger, rounder branch tips
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