Phenotypes Associated with This Genotype

Genotype
MGI:5486579

• Allelic Composition: Pax5^tm3Mbu/Pax5^tm3Mbu; Tg(Gad1-cre,-lacZ)1Mini/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:195054 )

• mice die 1-2 weeks after macroscopic changes appear (ie. domed skulls); life expectancy of such mutants is 58 days; mice that do not show this phenotype survive at least 12 months and are fertile

skeleton

domed cranium ( J:195054 )

• 47% of mice show dome shaped skulls by about postnatal day 50

craniofacial

domed cranium ( J:195054 )

• 47% of mice show dome shaped skulls by about postnatal day 50

behavior/neurological

increased anxiety-related response ( J:195054 )

• mutants spend less time in the center in open-field tests, suggesting increased anxiogenic-like behavior

increased locomotor activity ( J:195054 )

• in a novel open-field test, mutants with dome-shaped heads show significantly increased locomotion (likely due to the novelty aspect), as mutants appear lethargic in the home cages

nervous system

hydrocephaly ( J:195054 )

• 47% of mice

dilated lateral ventricle ( J:195054 )

• prominent, varying mild to severe

• in most severe cases, medial structures between ventricles are deformed and compressed, as represented by vertically relocated hippocampi

• ventricular dilation might be result of obstruction of interventricular connections, increased CSF production by ependymal cells of the choroid plexus, or decreased CSF resorption by arachnoid villi

• other ventricles do not show enlargement

abnormal cerebral cortex morphology ( J:195054 )

• cortex surrounding lateral ventricles is markedly thinner than in controls