Phenotypes Associated with This Genotype

Genotype
MGI:5427869

• Allelic Composition: Cdc42^tm1Brak/Cdc42^tm1Brak; Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• one week after tamoxifen administration, 56% of mutant crypts show stem cells forming a clustering pattern lacking clear demarcation between each other, while at 3 weeks after tamoxifen administration, 85% do so

• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity

• loss of stem cells

• intestinal crypt cells, most likely Paneth cells, contain large vacuoles

absent Paneth cells ( J:184563 )

• reduction or absence of Paneth cell granules, indicating loss of Paneth cells

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:184563 )

• one week after tamoxifen administration, 56% of mutant crypts show stem cells forming a clustering pattern lacking clear demarcation between each other, while at 3 weeks after tamoxifen administration, 85% do so

• stem cells in mutant crypts 3 weeks after tamoxifen treatment lose the typical triangle shapes and marker analysis indicates disrupted crypt cell polarity

• loss of stem cells

• intestinal crypt cells, most likely Paneth cells, contain large vacuoles

absent Paneth cells ( J:184563 )

• reduction or absence of Paneth cell granules, indicating loss of Paneth cells