Phenotypes Associated with This Genotype

Genotype
MGI:5319227

• Allelic Composition: Lepr^tm1.1Chua/Lepr^tm1.2Chua; Nos1^tm1(cre)Mgmj/Nos1⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body weight ( J:183754 )

• males have higher body weight relative to controls from 4-12 weeks similar to males with whole-body deletion of Lepr (Lepr^tm1.2Chua/ Lepr^tm1.2Chua; Nos1^tm1(cre)Mgmj/ Nos⁺ males)

• females display weight increase, but it is less severe than in females with whole-body deletion of Lepr

homeostasis/metabolism

decreased circulating thyroxine level ( J:183754 )

• levels of T4 are lower in males compared to controls; these levels are higher than in males with whole-body deletion of Lepr

increased circulating glucose level ( J:183754 )

• higher than controls from weaning age on in males and females

increased circulating corticosterone level ( J:183754 )

• circulating corticosterone levels in males are similar to controls

increased circulating insulin level ( J:183754 )

increased circulating leptin level ( J:183754 )

• starting at weaning age (8 weeks), much higher than controls at 8 weeks in males and females

decreased oxygen consumption ( J:183754 )

• males show decreased maximal oxygen consumption relative to controls at 12-14 weeks; oxygen consumption is similar to complete Lepr knockout males

adipose tissue

increased total body fat amount ( J:183754 )

• males have higher amount of adipose tissue similar to males with whole-body deletion of Lepr

behavior/neurological

behavior/neurological phenotype ( J:183754 )

N • circulating corticosterone levels are similar to controls

increased food intake ( J:183754 )

• from 4-12 weeks, males show increased food intake relative to controls similar to males with whole-body deletion of Lepr

• females display increased food consumption, but it is less severe than in females with whole-body deletion of Lepr

decreased locomotor activity ( J:183754 )

• at 12-14 weeks, males show decreased ambulatory activity in both dark and light cycles in 24-hour period

• females show decreased ambulatory activity during the light cycle

reproductive system

reproductive system phenotype ( J:183754 )

N • timing of litter delivery is similar to controls (24 days vs. 23 days in controls) and litter sizes are similar to controls

delayed estrous cycle ( J:183754 )

♀ • onset of vaginal estrous is delayed relative to controls in singly housed females