Phenotypes for Aicda Dicer1 MGI:5315120 MGI Mouse

immune system phenotype ( J:181782 )

N	• before immunization, mice have normal B-cell subsets in the bone marrow, spleen, lymph nodes, and peritoneal cavities

decreased germinal center B cell number ( J:181782 )

• significantly decreased numbers (6- to 10 fold) of germinal center (GC) B cells are present in the spleen at day 10 after immunization

• drastic reductions in GC B cell populations in Peyer patches and mesenteric lymph nodes are also observed

absent memory B cells ( J:181782 )

• NP-specific memory B (IgG1) cells are essentially absent from spleens of mice 56 days after primary immunization with NP-CGG

absent plasma cells ( J:181782 )

• NP-specific IgG1 antibody-secreting cells (ASCs) are absent from the bone marrow at 56 days after primary immunization with NP-CGG

abnormal germinal center B cell physiology ( J:181782 )

• at 10 days after immunization, numbers of proliferating GC B cells are 3- to 5-fold lower than in wild-type, suggesting impaired proliferative capacity

• GC B cells are mores susceptible to cell death; numbers of apoptotic cells are 30 to 50% higher than in controls at day 10 after immunization

abnormal humoral immune response ( J:181782 )

• upon immunization with a T cell dependent antigen (NP-CGG), mice display normal levels of NP-specific IgM antibodies at 14, 21, and 28 days following exposure, while NP-specific IgG1, IgGb and IgG3 antibody titers are drastically reduced

decreased immunoglobulin level ( J:181782 )

• mice have lower basal serum levels of IgG1, IgG2b, IgG3, and IgA compared to wild-type

• IgM level is normal