Phenotypes Associated with This Genotype

Genotype
MGI:5295219

• Allelic Composition: Pten^tm2Mak/Pten^tm2Mak; Tg(Hoxb7-cre)5526Cmb/0
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:122953 )

• a sharp decline in survival is noted after P15

• none of the mice survive beyond 26 days of life

growth/size/body

decreased body size ( J:122953 )

• at 2 wks of age, mice are readily identified by their smaller body size and sickly appearance

renal/urinary system

abnormal kidney collecting duct morphology ( J:122953 )

• neonatal kidneys display kinked collecting ducts

• DBA staining of collecting tubules within the cortex indicates higher density and cystic morphology

abnormal renal glomerulus morphology ( J:122953 )

• at P14, the density of glomeruli within the outermost cortical layer is significantly increased

• at P14, most glomeruli are small and unevenly positioned within the cortex (i.e. subcapsular)

abnormal glomerular capillary morphology ( J:122953 )

• at P14, aberrant subcapsular glomeruli show little evidence of capillary blood flow

impaired branching involved in ureteric bud morphogenesis ( J:122953 )

• in vitro, E11.5 kidneys cultured for 48 hrs in serum free media display subtle branching defects including asymmetric ureteric bud tips that are often dilated, abnormally enlarged misshapen ampullae, discontinuous and jagged laminin basement membranes, kinked ureteric bud stalks and small ectopic bud outgrowths

• neonatal kidneys exhibit terminal end buds that are often dilated and connected to misshaped stalks

ectopic ureteric bud ( J:122953 )

• in vitro, E11.5 kidneys cultured for 48 hrs in serum free media display small ectopic bud outgrowths

cardiovascular system

abnormal glomerular capillary morphology ( J:122953 )

• at P14, aberrant subcapsular glomeruli show little evidence of capillary blood flow