Phenotypes Associated with This Genotype

Genotype
MGI:4939899

• Allelic Composition: Cd47^tm1Fpl/Cd47^tm1Fpl
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

respiratory system

pleural effusion ( J:134866 )

• accumulation of excess fluid in the lungs 24 hours after intratracheal instillation with LPS or after lung infection with E. coli is reduced

• bronchoalveolar lavage protein concentrations reduced

hematopoietic system

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

decreased hematocrit ( J:134866 )

• relative to controls after intratracheal instillation with LPS or after lung infection with E. coli

decreased hemoglobin content ( J:134866 )

• relative to controls after intratracheal instillation with LPS or after lung infection with E. coli

decreased leukocyte cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS

decreased neutrophil cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS or after lung infection with E. coli

skeleton

abnormal bone structure ( J:147367 )

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

increased trabecular bone volume ( J:147367 )

• by microCT analysis

increased trabecular bone thickness ( J:147367 )

• thickness but not number is increased in tibias

abnormal skeleton physiology ( J:147367 )

• reduced cleavage of type I collagen

decreased bone resorption ( J:147367 )

• osteoclast bone resorption is reduced unless RANKL concentration is elevated

cardiovascular system

increased heart rate ( J:148948 )

• in active light cycle

increased mean systemic arterial blood pressure ( J:148948 )

• increased 15% relative to controls under isofluorane anaesthesia

increased systemic arterial diastolic blood pressure ( J:148948 )

• in active light cycle

• peripheral diastolic pressure is significantly increased

increased systemic arterial systolic blood pressure ( J:148948 )

• peripheral systolic blood pressure is significantly increased

integument

decreased sensitivity to skin irradiation ( J:139652 )

• hair is preserved after 25 Gy irradiation

• minimal or no skin ulceration 8 weeks after irradiation

• hair follicles and skin architecture are better preserved than in controls

muscle

muscle phenotype ( J:139652 )

N • no leg muscle atrophy after irradiation

N • limb flexibility maintained

abnormal muscle fiber morphology ( J:139652 )

• less loss of muscle fibers and nuclei after irradiation

homeostasis/metabolism

pleural effusion ( J:134866 )

• accumulation of excess fluid in the lungs 24 hours after intratracheal instillation with LPS or after lung infection with E. coli is reduced

• bronchoalveolar lavage protein concentrations reduced

abnormal cytokine level ( J:134866 )

• MIP-2 levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

abnormal tumor necrosis factor level ( J:134866 )

• levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

immune system

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

decreased leukocyte cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS

decreased neutrophil cell number ( J:134866 )

• significantly reduced relative to controls in both blood and bronchoalveolar lavage after intratracheal instillation with LPS or after lung infection with E. coli

abnormal cytokine level ( J:134866 )

• MIP-2 levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

abnormal tumor necrosis factor level ( J:134866 )

• levels in bronchoalveolar fluid are reduced 4 hours after LPS challenge but not after 24 hours

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:134866 )

• enhanced survival 72 hours after E. coli infection with 5 x 10 ⁶ CFU

increased susceptibility to bacterial infection ( J:134866 )

• after E coli infection of the lungs, bacterial levels remain high in the lungs but are reduced in the blood

neoplasm

decreased tumor incidence ( J:147367 )

• fewer tumors develop in femur, tibia and mandible from B16-F10 melanoma cell injection

mortality/aging

decreased susceptibility to bacterial infection induced morbidity/mortality ( J:134866 )

• enhanced survival 72 hours after E. coli infection with 5 x 10 ⁶ CFU

cellular

abnormal osteoclast differentiation ( J:147367 )

• macrophage differentiation into osteoclasts is severely reduced in culture

• increased doses of RANKL increases osteoclast formation

nervous system

nervous system phenotype ( J:204011 )

N • exhibit normal neurite differentiation