Cd47^tm1Fpl
Targeted Allele Detail

Summary

• Symbol: Cd47^tm1Fpl
• Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer); targeted mutation 1, Frederik P Lindberg
• MGI ID: MGI:1861955
• Synonyms: CD47^-, IAP^-, Itgp^tm1Fpl
• Gene: Cd47 Location: Chr16:49671691-49732799 bp, + strand Genetic Position: Chr16, 31.23 cM
• Alliance: Cd47^tm1Fpl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36489
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 2 of the gene, which encodes the signal peptide cleavage site and the IgV domain. Monoclonal antibodies recognizing the Ig domain failed to detect any CD47 antigen on the surface of erythrocytes, bone marrow cells, or other cells including peripheral blood leukocytes, splenocytes, and fibroblasts from homozygous mutant mice. In addition, no antigen was detected in mutant erythrocyte lysates with polyclonal antisera to the NH2 or COOH-termini. (J:36489)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Cd47 Mutation: 45 strains or lines available

References

• Original: J:36489 Lindberg FP, et al., Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice. Science. 1996 Nov 1;274(5288):795-8
• All: 108 reference(s)