Mouse Genome Informatics
cn
    Casp8tm1Hed/Casp8tm1Hed
Tg(KRT14-cre)1Efu/0

Not Specified
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
       
mortality/aging
• about 90% of mutants die by P15, while 10% survive for months
• about 10% of mutants exhibit a mosaic skin phenotype and survive for months

integument
• mutants exhibit an increase in epidermal water loss
• mutants develop atopic dermatitis-like skin disease
• about 90% of mutants exhibit a uniform skin phenotype and die by P15 while mutants that survive longer than P15, exhibit the skin phenotype on the posterior region of the back skin
• 2.5-fold increase in the numbers of blood vessels in the skin as indicated by marker analysis
• cutaneous edema is seen in the ears and feet
• affected skin of mutants surviving longer than P15 shows suprabasal cells with increased intracellular space, indicating development of spongiosis
• hyperproliferation of epithelial stem/progenitor cells in the skin
• the intracellular adhesion apparatus of the epidermis is disrupted, with keratinocytes showing an abnormal punctate localization of E-cadherin (Cdh1) due to increased shedding of E-cadherin
• mutants exhibit epidermal hyperplasia
• topical application of clobetasol, a corticosteroid, substantially reduces the epidermal hyperplasia and abolishes the epidermal gaps

immune system
• mutants exhibit an increase in mast cells with age
• IgE levels are normal in young mutants but adults show a 30-fold increase compared to wild-type mice
• 5-fold increase in serum IgG1 levels in adults
• marker analysis indicates that mutants exhibit a biphastic T-helper cell response, with a TH2 response during the acute phase of dermatitis followed by a TH1 response during the chronic phase of dermatitis
• mutants develop atopic dermatitis-like skin disease
• about 90% of mutants exhibit a uniform skin phenotype and die by P15 while mutants that survive longer than P15, exhibit the skin phenotype on the posterior region of the back skin

homeostasis/metabolism
• cutaneous edema is seen in the ears and feet
• affected skin of mutants surviving longer than P15 shows suprabasal cells with increased intracellular space, indicating development of spongiosis
• mutants exhibit an increase in epidermal water loss

hematopoietic system
• mutants exhibit an increase in mast cells with age
• IgE levels are normal in young mutants but adults show a 30-fold increase compared to wild-type mice
• 5-fold increase in serum IgG1 levels in adults
• marker analysis indicates that mutants exhibit a biphastic T-helper cell response, with a TH2 response during the acute phase of dermatitis followed by a TH1 response during the chronic phase of dermatitis

cardiovascular system
• 2.5-fold increase in the numbers of blood vessels in the skin as indicated by marker analysis

Mouse Models of Human Disease
OMIM IDRef(s)
Dermatitis, Atopic 603165 J:167299