mortality/aging
• mutants do not survive as newborns
|
growth/size/body
• at E19.5, mutant fetuses are smaller than control fetuses
|
hematopoietic system
• at E17.5, mutant fetuses display severe hemolytic anemia
|
• at E17.5, mutant erythrocytees display notable anisocytosis, poikilocytosis, polychromasia, and targeting, characteristic of severe thalassemia
• virtually all mutant erythrocytes exhibit hemoglobin H inclusions
|
• at E17.5, hemoglobin H (beta4) is the major component constituting 55%-65% of total hemoglobin, as shown by isoelectric focusing electrophoresis
|
anisocytosis
(
J:35018
)
leptocytosis
(
J:35018
)
• at E17.5, mutant fetuses exhibit very high reticulocyte counts
|
integument