Phenotypes Associated with This Genotype

Genotype
MGI:4452405

• Allelic Composition: Dicer1^tm1Mmk/Dicer1^tm1Mmk; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0
• Genetic Background: involves: 129 * C57BL/6 * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased tumor-free survival time ( J:158901 )

• mice with tumors die by 4 to 5 weeks of age

• 30% of mice die by 8 weeks of age

craniofacial

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

hematopoietic system

hematopoietic system phenotype ( J:158901 )

N • mice exhibit normal frequency and function of hematopoietic stem cells and progenitors

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

extramedullary hematopoiesis ( J:158901 )

• in the spleen of some mice

anemia ( J:158901 )

• profound in some mice

impaired hematopoiesis ( J:158901 )

• hematopoietic progenitor cells exhibit increased apoptosis and proliferation compared with wild-type cells

• megakaryocyte-erythroid progenitor cells exhibit increased apoptosis compared to in wild-type mice

• wild-type mice transplanted with transgenic hematopoietic cells exhibit cytopenia and myelodysplastic features

• however, differentiated cells exhibit normal apoptosis

increased bone marrow cell number ( J:158901 )

• in some mice

abnormal megakaryocyte morphology ( J:158901 )

• micro-megakaryocytes with hypolobulated, hyperchromatic nuclei in the bone marrow

abnormal megakaryocyte differentiation ( J:158901 )

• impaired

thrombocytopenia ( J:158901 )

• profound in some mice

giant platelets ( J:158901 )

• giant platelets in the blood

decreased leukocyte cell number ( J:158901 )

• with all subsets of leukocytes exhibiting decreased numbers

decreased B cell number ( J:158901 )

• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice

abnormal myeloid leukocyte morphology ( J:158901 )

• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice

abnormal granulocyte differentiation ( J:158901 )

abnormal neutrophil morphology ( J:158901 )

• hypersegementation

skeleton

abnormal bone structure ( J:158901 )

• texture of mineralized is altered compared to in wild-type mice

• however, trabecular and cortical bone volumes are normal

abnormal bone marrow morphology ( J:158901 )

• bone marrow vascularity is increased compared to in wild-type mice

decreased osteoblast cell number ( J:158901 )

• mildly

abnormal skeleton development ( J:158901 )

• mice exhibit reduced bone marrow stromal osteogenic colony numbers relative to total colony forming numbers compared with wild-type mice

• bone marrow stromal cultured exhibit reduced alkaline phosphatase and calcified matrix deposition compared with wild-type cultures

neoplasm

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

increased hemolymphoid system tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop myeoblastic tumors

increased leukemia incidence ( J:158901 )

• mice exhibit sporadic acute myelogenous leukemia unlike wild-type mice

increased myeloid sarcoma incidence ( J:158901 )

• mice exhibit sporadic myeloid sarcoma with infiltration behavior unlike wild-type mice

cardiovascular system

abnormal cardiovascular system morphology ( J:158901 )

• bone marrow vascularity is increased compared to in wild-type mice

growth/size/body

increased facial tumor incidence ( J:158901 )

• at 2 to 3 weeks of age, mice develop facial tumors

postnatal growth retardation ( J:158901 )

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

immune system

enlarged spleen ( J:158901 )

• mice with myeloid sarcomas exhibit increased spleen weight with blast cell infiltration unlike in wild-type mice

decreased leukocyte cell number ( J:158901 )

• with all subsets of leukocytes exhibiting decreased numbers

decreased B cell number ( J:158901 )

• B cell and B cell progenitors are decreased in the bone marrow compared to in wild-type mice

abnormal myeloid leukocyte morphology ( J:158901 )

• myeloid cell frequency is increased in the bone marrow compared to in wild-type mice

abnormal granulocyte differentiation ( J:158901 )

abnormal neutrophil morphology ( J:158901 )

• hypersegementation

cellular

abnormal granulocyte differentiation ( J:158901 )

abnormal megakaryocyte differentiation ( J:158901 )

• impaired