Phenotypes for Tg(SFTPC-rtTA,tetO-Tnf)320-1Gwho MGI:4437866 MGI Mouse

lung inflammation ( J:97297 )

• lymphocytes in the lung in doxycycline-treated transgenic mice

• clustered inflammatory cells within lymphocytic nodules, within the parenchyma, and adjacent to the pleural surface

• varying degrees of inflammation in untreated transgenic mice, but overall much less pronounced than in doxycycline-treated transgenic mice

emphysema ( J:97297 )

• enlarged airspace throughout the lung in transgenic mice treated with doxycycline

• tend to be more pronounced adjacent to lymphoid aggregates

• significant increases in linear intercept in transgenic mice treated with doxycycline for 1-9 months

• significant airspace enlargement at 11-12 months of age in untreated transgenic mice

pulmonary fibrosis ( J:97297 )

• some fibrotic lesions in transgenic mouse treated with doxycycline for 6 months

• most commonly adjacent to airways

increased neutrophil cell number ( J:97297 )

• modest increases in the number of neutrophils recovered in lavage fluid (6% of total cells) in doxycycline-treated transgenic mice

increased lymphocyte cell number ( J:97297 )

• modest increases in the number of lymphocytes recovered in lavage fluid (2% of total cells) in doxycycline-treated transgenic mice

abnormal dendritic cell morphology ( J:97297 )

• dendritic cells on the periphery of lymphoid nodules as shown by CD11c immunostaining in doxycycline-treated transgenic mice

abnormal B cell morphology ( J:97297 )

• large numbers of B lymphocytes within lymphoid nodules in transgenic mice administered doxycycline for 1-2 months as shown by B220 immunostaining

abnormal T cell morphology ( J:97297 )

• T cell-rich zones in some lymphoid nodules as shown by CD3 immunostaining in doxycycline-treated transgenic mice

increased CD8-positive, alpha-beta T cell number ( J:97297 )

• increased CD8-positive cells within the lung parenchyma in the transgenic mice administered doxycycline for 1-2 months

abnormal CD4-positive, alpha beta T cell morphology ( J:97297 )

• CD4-positive cells in some nodules but no CD8-positive cells in doxycycline-treated transgenic mice

abnormal bronchus-associated lymphoid tissue morphology ( J:97297 )

• lymphocytic nodules and enlarged airspaces in the lung of transgenic mice treated with doxycycline for 1 month

• significantly higher number of lymphocytic nodules in doxycycline treated transgenic mice than in untreated transgenic mice

• small to medium sized lymphocytic nodule but normal airspaces in some untreated transgenic mouse of same age

• no inflammatory nodules observed in nontransgenic mice