Phenotypes Associated with This Genotype

Genotype
MGI:3851980

• Allelic Composition: Il21r^tm1Day/Il21r^tm1Day
• Genetic Background: involves: BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Impaired allergic skin inflammation and systemic response to epicutaneous sensitization in Il21r^tm1Day/Il21r^tm1Day mice

immune system

abnormal CD4-positive T cell differentiation ( J:123352 , J:136955 )

• the frequency of Th17 cells induced from nave T cells by Tgf-beta + IL-6 incubation is half that of controls (J:123352)

• IL-21 fails to differentiate nave T cells under Th2 polarizing conditions into IL-21 secreting effector T cells (J:136955)

decreased CD4-positive, alpha-beta T cell number ( J:123352 )

• the fraction of CD4⁺CD44⁺IL-17⁺ T cells is severely reduced compared to controls

decreased IgE level ( J:144708 )

• levels of OVA-specific IgE are severely diminished compared to controls after immunization through the skin

increased IgE level ( J:76043 )

• IgE levels are increased 3-fold

decreased IgG1 level ( J:76043 , J:144708 )

• IgG1 levels are decreased 3-fold (J:76043)

• mice fail to make OVA-specific IgG1 in response to immunization through the skin (J:144708)

decreased IgG2a level ( J:144708 )

• mice fail to make OVA-specific IgG2a in response to immunization through the skin

decreased IgG2b level ( J:76043 )

• IgG2b levels are decreased 2-fold

abnormal NK cell physiology ( J:76043 )

• IL-21 fails to inhibit IL-15-mediated expansion of NK cells as it does with wild-type NK cells

• IL-21 fails to enhance IL-12 mediated secretion of IFN-gamma by activated NK cells

impaired natural killer cell mediated cytotoxicity ( J:76043 )

• IL-21 fails to enhance cytolytic activity of activated NK cells as it does with wild-type NK cells

abnormal T cell physiology ( J:76043 )

• IL-21 fails to enhance IFN-gamma secretion mediated by TCR stimulation in the presence of IL-2 or IL-15

decreased cytotoxic T cell cytolysis ( J:76043 )

• IL-21 fails to enhance cytolytic activity of T cells in response to alloantigen

abnormal T cell activation ( J:76043 )

• IL-21 does not enhance alloantigen stimulation of T cells

abnormal thymocyte activation ( J:76043 )

• thymocytes fail to respond to sub-optimal doses of CD3 and IL-21

abnormal memory T cell physiology ( J:76043 )

• IL-21 fails to inhibit IL-15-mediated expansion of memory CD8⁺ T cells as it does with wild-type NK cells

abnormal dendritic cell physiology ( J:144708 )

• myeloid cells have defects in migration to draining lymph nodes after activation in the skin

abnormal Langerhans cell physiology ( J:144708 )

• Langerhans cells have defects in migration to draining lymph nodes after activation in the skin

abnormal plasmacytoid dendritic cell physiology ( J:144708 )

• plasmacytoid cells have defects in migration to draining lymph nodes after activation in the skin

decreased susceptibility to type IV hypersensitivity reaction ( J:144708 )

• mice are resistant to atopic dermatitis induced by OVA sensitization on skin stripped of hair

• mice also mount a significantly diminished cutaneous hypersensitivity response to skin painting with the hapten oxazolone

• mice also fail to mount systemic response to OVA antigen when sensitized through the skin as evidence by lack of cytokine production of splenocytes to OVA

decreased interferon-gamma secretion ( J:144708 )

• splenocytes from mice sensitized to OVA through the skin fail to produce IFN-gamma upon OVA stimulation

increased interferon-gamma secretion ( J:76043 , J:123352 )

• IL-21 fails to enhance IL-12 mediated secretion of IFN-gamma by activated NK cells (J:76043)

• IL-21 fails to enhance IFN-gamma secretion mediated by TCR stimulation in the presence of IL-2 or IL-15 (J:76043)

• more IFN-gamma is secreted when T cells from OVA-challenged mice are presented OVA peptide in vitro (J:123352)

decreased interleukin-13 secretion ( J:144708 )

• splenocytes from mice sensitized to OVA through the skin fail to produce IL-13 upon OVA stimulation

decreased interleukin-17 secretion ( J:123352 )

• less IL-17 is secreted when T cells from OVA-challenged mice are presented OVA peptide in vitro

decreased interleukin-4 secretion ( J:144708 )

• splenocytes from mice sensitized to OVA through the skin fail to produce IL-4 upon OVA stimulation

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:123352 , J:136955 )

• the frequency of Th17 cells induced from nave T cells by Tgf-beta + IL-6 incubation is half that of controls (J:123352)

• IL-21 fails to differentiate nave T cells under Th2 polarizing conditions into IL-21 secreting effector T cells (J:136955)

decreased CD4-positive, alpha-beta T cell number ( J:123352 )

• the fraction of CD4⁺CD44⁺IL-17⁺ T cells is severely reduced compared to controls

decreased IgE level ( J:144708 )

• levels of OVA-specific IgE are severely diminished compared to controls after immunization through the skin

increased IgE level ( J:76043 )

• IgE levels are increased 3-fold

decreased IgG1 level ( J:76043 , J:144708 )

• IgG1 levels are decreased 3-fold (J:76043)

• mice fail to make OVA-specific IgG1 in response to immunization through the skin (J:144708)

decreased IgG2a level ( J:144708 )

• mice fail to make OVA-specific IgG2a in response to immunization through the skin

decreased IgG2b level ( J:76043 )

• IgG2b levels are decreased 2-fold

abnormal NK cell physiology ( J:76043 )

• IL-21 fails to inhibit IL-15-mediated expansion of NK cells as it does with wild-type NK cells

• IL-21 fails to enhance IL-12 mediated secretion of IFN-gamma by activated NK cells

impaired natural killer cell mediated cytotoxicity ( J:76043 )

• IL-21 fails to enhance cytolytic activity of activated NK cells as it does with wild-type NK cells

abnormal T cell physiology ( J:76043 )

• IL-21 fails to enhance IFN-gamma secretion mediated by TCR stimulation in the presence of IL-2 or IL-15

decreased cytotoxic T cell cytolysis ( J:76043 )

• IL-21 fails to enhance cytolytic activity of T cells in response to alloantigen

abnormal T cell activation ( J:76043 )

• IL-21 does not enhance alloantigen stimulation of T cells

abnormal thymocyte activation ( J:76043 )

• thymocytes fail to respond to sub-optimal doses of CD3 and IL-21

abnormal memory T cell physiology ( J:76043 )

• IL-21 fails to inhibit IL-15-mediated expansion of memory CD8⁺ T cells as it does with wild-type NK cells

endocrine/exocrine glands

abnormal thymocyte activation ( J:76043 )

• thymocytes fail to respond to sub-optimal doses of CD3 and IL-21