Phenotypes Associated with This Genotype

Genotype
MGI:3843276

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Slc3a2^tm1.1Mgin/Slc3a2^tm1.1Mgin
• Genetic Background: involves: 129 * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:148000 )

N • despite Cre mediated deletion in B cells, mice have normal pre-B, pro-B, immature and mature B cell numbers and normal splenic architecture

abnormal plasma cell differentiation ( J:148000 )

• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells

• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal

decreased germinal center B cell number ( J:148000 )

• decrease in germinal center B cell and plasma cell numbers 1 week after immunization

• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers

abnormal B cell physiology ( J:148000 )

• impaired antibody secretion following LPS stimulation

abnormal class switch recombination ( J:148000 )

• impaired class switching following LPS stimulation

abnormal B cell activation ( J:148000 )

• activated B cells fail to spread after antibody mediated ligation of integrins

decreased B cell proliferation ( J:148000 )

• minimal proliferation in response to anti-IgM antibody stimulation

• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells

decreased IgG level ( J:148000 )

• decrease in basal and antigen specific levels of IgG

decreased IgM level ( J:148000 )

• decrease in antigen specific levels of IgM

• however, basal IgM levels are similar to controls

hematopoietic system

abnormal plasma cell differentiation ( J:148000 )

• defect in the ability of LPS stimulated isolated B cells to differentiate into plasma cells

• however, in a small percentage of cells where Cre mediated recombination did not take place plasma cell differentiation is normal

decreased germinal center B cell number ( J:148000 )

• decrease in germinal center B cell and plasma cell numbers 1 week after immunization

• however, by 2 - 3 weeks after immunization antigen specifc antibody titers are similar to controls suggesting strong selection in germinal centers for the few cells that escaped Cre mediated recombination and expression analysis confirms the presence of B cells expressing SLC3A2 in the germinal centers

abnormal B cell physiology ( J:148000 )

• impaired antibody secretion following LPS stimulation

abnormal class switch recombination ( J:148000 )

• impaired class switching following LPS stimulation

abnormal B cell activation ( J:148000 )

• activated B cells fail to spread after antibody mediated ligation of integrins

decreased B cell proliferation ( J:148000 )

• minimal proliferation in response to anti-IgM antibody stimulation

• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells

decreased IgG level ( J:148000 )

• decrease in basal and antigen specific levels of IgG

decreased IgM level ( J:148000 )

• decrease in antigen specific levels of IgM

• however, basal IgM levels are similar to controls

cellular

decreased B cell proliferation ( J:148000 )

• minimal proliferation in response to anti-IgM antibody stimulation

• in vivo, B cells carrying the recombined allele fail to proliferate; however, cells that escape Cre mediated recombination do proliferate and go on to form plasma cells