Phenotypes for Kmt2a Tg(GFAP-cre)25Mes MGI:3839880 MGI Mouse

premature death ( J:147104 )

• mice die between P25 and P30

abnormal astrocyte morphology ( J:147104 )

• the subventricular zone and other brain regions contains more cells positive for an astrocyte-specific marker than in wild-type mice

abnormal neuron differentiation ( J:147104 )

• cultured subventricular cells produce fewer neuronal cells while remaining gliogenic unlike wild-type cells

• however, cultured subventricular cells exhibit normal proliferation and apoptosis

abnormal neuronal precursor cell migration ( J:147104 )

• neuroblast chain migration in mice and from subventricular zone explants is severely disorganized, and migration is reduced 40% compared to wild-type cells

abnormal brain development ( J:147104 )

• all regions of the brain that undergo postnatal neurogenesis including the cerebellar granule cell layer, hippocampal dentate gyrus, and olfactory bulb are reduced in size compared to in wild-type mice

• however, the ependymal layer and oligodendrocytes develop normally

abnormal dentate gyrus morphology ( J:147104 )

• reduced in size with reduced neurons

small olfactory bulb ( J:147104 )

• with reduced neurons

thin cerebellar granule layer ( J:147104 )

• with reduced neurons

abnormal postnatal subventricular zone morphology ( J:147104 )

• the expanded subventricular zone contains 3- to 4-fold fewer proliferating neuroblasts compared to in wild-type mice

• after P7, neuroblasts accumulate in the subventricular zone due to impaired migration unlike in wild-type mice

• the subventricular zone contains more cells positive for an astrocyte-specific marker than in wild-type mice

decreased neuronal precursor cell number ( J:147104 )

• the subventricular zone contains 3- to 4-fold fewer proliferating neuroblasts compared to in wild-type mice

• under differentiation condition, cultured subventricular zone cells produce more than 20-fold fewer Tuj1+ (marking neuron precursor cells) compared similarly treated wild-type cells

ataxia ( J:147104 )

postnatal growth retardation ( J:147104 )

• progressive