Phenotypes for Pou4f2 Tg(Six3-cre)69Frty MGI:3838794 MGI Mouse

vision/eye

abnormal optic nerve morphology ( J:97089 )

• clearly abnormal in 16 day old mice

• thinner with smaller diameter fibers

• unmyelinated fibers

abnormal optic disk morphology ( J:97089 )

• deformed at E14.5

abnormal retina morphology ( J:97089 )

• overall structure remains normal through 5 months of age

• reduced cell proliferation and increased apoptosis

thin retina ganglion layer ( J:97089 )

• more than 98% of retinal ganglion cells ablated at E14.5

• only displaced amacrine cells present at 16 days of age

decreased total retina thickness ( J:97089 )

• noticeably thinner at E14.5

• 30-50% thinner than controls at 16 days of age

abnormal eye electrophysiology ( J:97089 )

• saturated amplitudes of both light and dark adapted b-waves 25% of normal

• dark adapted a-wave amplitude 42% of normal

• negative scotopic threshold response is very small

• positive scotopic threshold response is absent

nervous system