Phenotypes Associated with This Genotype

Genotype
MGI:3834909

• Allelic Composition: \Tg(LCK-NFKBIA)5Dwb/0
• Genetic Background: involves: C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell apoptosis ( J:93003 )

• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A

• this is observed for both CD4⁺ and CD8⁺ T cells

• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8⁺ T cells

decreased T cell proliferation ( J:93003 )

• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin

• thymocyte proliferation is also severely curtailed in response to other mitogens

• addition of IL-2 to culture does not rescue the proliferation defect

• similar results were observed for B cell-depleted splenocytes

abnormal CD8-positive, alpha-beta T cell differentiation ( J:93003 )

• numbers of CD8 single-positive thymocytes bearing high levels of TCR are reduced by 40% compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:93003 )

• CD4⁺ T cell numbers are reduced in the spleen, lymph nodes, and blood though not to the degree of reduction observed for CD8⁺ T cells

decreased CD8-positive, alpha-beta T cell number ( J:93003 )

• CD8⁺ T cell numbers are reduced by two-thirds or more in the spleen, lymph nodes, and blood

decreased interleukin-2 secretion ( J:93003 )

• thymocytes produce about 20-fold less IL-2 when stimulated compared to wild-type controls

• likewise, splenocytes only produce 8% the IL-2 as controls when stimulated

hematopoietic system

increased T cell apoptosis ( J:93003 )

• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A

• this is observed for both CD4⁺ and CD8⁺ T cells

• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8⁺ T cells

decreased T cell proliferation ( J:93003 )

• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin

• thymocyte proliferation is also severely curtailed in response to other mitogens

• addition of IL-2 to culture does not rescue the proliferation defect

• similar results were observed for B cell-depleted splenocytes

abnormal CD8-positive, alpha-beta T cell differentiation ( J:93003 )

• numbers of CD8 single-positive thymocytes bearing high levels of TCR are reduced by 40% compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:93003 )

• CD4⁺ T cell numbers are reduced in the spleen, lymph nodes, and blood though not to the degree of reduction observed for CD8⁺ T cells

decreased CD8-positive, alpha-beta T cell number ( J:93003 )

• CD8⁺ T cell numbers are reduced by two-thirds or more in the spleen, lymph nodes, and blood

cellular

increased T cell apoptosis ( J:93003 )

• about twice the percentage of T cells are apoptotic after in vitro stimulation with Con-A

• this is observed for both CD4⁺ and CD8⁺ T cells

• anti-TCR stimulation produces similar results with more enhanced apoptosis observed for CD8⁺ T cells

decreased T cell proliferation ( J:93003 )

• thymocyotes only have one third the proliferation rate as controls when stimulated with PMA and ionomycin

• thymocyte proliferation is also severely curtailed in response to other mitogens

• addition of IL-2 to culture does not rescue the proliferation defect

• similar results were observed for B cell-depleted splenocytes