Phenotypes Associated with This Genotype

Genotype
MGI:3818431

• Allelic Composition: Il4^tm1.1Wep/Il4^tm1.1Wep; Stat6^tm1Gru/Stat6^tm1Gru
• Genetic Background: C.129-Stat6^tm1Gru Il4^tm1.1Wep

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced

immune system

abnormal T-helper 2 cell differentiation ( J:138827 )

• CD4⁺ T cells fail to express IL-4 or GFP after in vitro activation under Th2 conditions

• these in vitro activated T cells also fail to upregulate mRNA encoding for IL-13, IL-5, IL-10, and GATA-3 proteins

• however, in vivo Th2 cell differentiation in response to a parasitic nematode infection is normal with Th2 cell numbers peaking at days 9-12 after primary infection and days 5-7 after secondary infection

• these Th2 cells upregulate mRNA encoding for IL-13, IL-5, and GATA-3 proteins

• normal in vivo Th2 cell differentiation is also observed in the Th2 priming models of allergic airway using either keyhole limpet hemocyanin or OVA sensitization, IgD neutralization, and intradermal ear injection of dead nematodes

impaired eosinophil recruitment ( J:138827 )

• eosinophil recruitment to the airways does not occur during infection with a parasitic nematode compared to controls which has a sizable migration for the first two weeks after primary and secondary infections

decreased IgE level ( J:138827 )

• serum IgE is absent 15 days after parasitic nematode infection compared to the 400 ng/ml present in controls

• serum IgE is also absent when the allergic airway model or the IgD neutralization model is induced