Analysis Tools
mortality/aging
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• less mice die 48 hours after sepsis induction by cecal ligation and puncture than in control
• 40% of mice die compared to 10% of wild-type mice subjected to the same sepsis model
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immune system
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly decreased levels of IL-10 four hours after administration compared to controls
• less IL-10 is also found in the plasma of mice undergoing sepsis from cecal ligation and puncture
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-12a four hours after administration compared to controls
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• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-6 four hours after administration compared to controls
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of TNF one hour after administration compared to controls
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• LPS stimulation of bone marrow derived macrophages leads to decreased secretion of IL-10 than in controls during an 8 hour time period
• the discrepancy is greater during the early time points; 8 hours after stimulation secretion levels have reached 60% of wild-type
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• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12a than in controls during an 8 hour time period
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• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period
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• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL-6 than in controls during an 8 hour time period
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• stimulation of bone marrow derived macrophages leads to higher secretion of TNF than in controls during an 8 hour time period
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• 2.5 mg/kg bodyweight causes LPS-induced toxicity in these mice while control mice are normal
• signs of toxicity include diarrhea, eye inflammation, piloerection, and a substantial drop in temperature within 4-8 hours of LPS injection
• mice were humanly sacrificed within 8 hours of LPS injection due to severity of symptoms
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• inflammation fails to resolve in phorbol 12-myristate 13-acetate (PMA) induced skin eczema of the ears
• ears of wild-type mice exposed to PMA swell from inflammatory infiltrate but resolve this swelling within 96 hours while mutant mice still have significant swelling 96 hours after PMA administration
• myeloperoxidase activity of ear extracts, a measure of polymorphonuclear cell recruitment, is significantly higher than controls both 72 and 96 hours after PMA administration
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homeostasis/metabolism
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly decreased levels of IL-10 four hours after administration compared to controls
• less IL-10 is also found in the plasma of mice undergoing sepsis from cecal ligation and puncture
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-12a four hours after administration compared to controls
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• LPS stimulation of bone marrow derived macrophages leads to higher secretion of IL12b than in controls during an 8 hour time period
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of IL-6 four hours after administration compared to controls
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• LPS administration (1.8 mg/kg bodyweight) leads to significantly increased levels of TNF one hour after administration compared to controls
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integument
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• inflammation fails to resolve in phorbol 12-myristate 13-acetate (PMA) induced skin eczema of the ears
• ears of wild-type mice exposed to PMA swell from inflammatory infiltrate but resolve this swelling within 96 hours while mutant mice still have significant swelling 96 hours after PMA administration
• myeloperoxidase activity of ear extracts, a measure of polymorphonuclear cell recruitment, is significantly higher than controls both 72 and 96 hours after PMA administration
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