Phenotypes Associated with This Genotype

Genotype
MGI:3800764

• Allelic Composition: Cd4^tm1Mak/Cd4^tm1Mak; Cd8a^tm1Mak/Cd8a^tm1Mak
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

small thymus medulla ( J:12572 )

• the medulla is reduced in size

abnormal T cell differentiation ( J:12572 )

• there is a 5-fold drop in the number of mature alphabeta TCR⁺ HAS^- T cells in the thymus

• alphabeta TCR expression on these mature double negative cells is lower than in mature T cells of wild-type mice

• despite lack of CD4 and CD8 expression, alphabeta T cells are still present in the periphery

abnormal double-negative T cell morphology ( J:12572 )

• "mature" double negative thymocytes are found that are alphabeta TCR⁺ HAS^-

decreased double-positive T cell number ( J:12572 )

• double positive T cells are absent

abnormal T cell number ( J:12572 )

• the alphabeta T cell number in lymph nodes varies from 5% to 50% of normal

abnormal gamma-delta T cell number ( J:12572 )

• the absolute number of gammadelta T cells is decreased though the relative percentage of lymph node is increased from 1% to 3%

decreased cytotoxic T cell cytolysis ( J:12572 )

• LCMV-specific class I MHC-restricted cytotoxic T cell responses are not activated in these mice

• alloantigen specific lysis of target cells by T cells in mixed lymphocyte culture is reduced

• the lysis of cells is specific for class I MHC antigens

increased length of allograft survival ( J:12572 )

• tail skin graphs from donor mice of the same MHC haplotypes are not rejected within a three month period compared to controls that have no graph survival after 18 days

• when donor mice have a different MHC haplotype, skin graft rejection is similar to controls

hematopoietic system

small thymus medulla ( J:12572 )

• the medulla is reduced in size

abnormal T cell differentiation ( J:12572 )

• there is a 5-fold drop in the number of mature alphabeta TCR⁺ HAS^- T cells in the thymus

• alphabeta TCR expression on these mature double negative cells is lower than in mature T cells of wild-type mice

• despite lack of CD4 and CD8 expression, alphabeta T cells are still present in the periphery

abnormal double-negative T cell morphology ( J:12572 )

• "mature" double negative thymocytes are found that are alphabeta TCR⁺ HAS^-

abnormal T cell number ( J:12572 )

• the alphabeta T cell number in lymph nodes varies from 5% to 50% of normal

decreased double-positive T cell number ( J:12572 )

• double positive T cells are absent

abnormal gamma-delta T cell number ( J:12572 )

• the absolute number of gammadelta T cells is decreased though the relative percentage of lymph node is increased from 1% to 3%

decreased cytotoxic T cell cytolysis ( J:12572 )

• LCMV-specific class I MHC-restricted cytotoxic T cell responses are not activated in these mice

• alloantigen specific lysis of target cells by T cells in mixed lymphocyte culture is reduced

• the lysis of cells is specific for class I MHC antigens

endocrine/exocrine glands

small thymus medulla ( J:12572 )

• the medulla is reduced in size