Phenotypes Associated with This Genotype

Genotype
MGI:3799693

• Allelic Composition: Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(TcrHEL3A9)1Mmd/0; Tg(TLK2mHEL)2Ccg/0
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

endocrine/exocrine glands

thyroid gland inflammation ( J:78309 )

• 54% of mice develop thyroiditis compared to 100% of mice carrying both transgenes on a Rag2⁺ background

hematopoietic system

decreased double-positive T cell number ( J:78309 )

• the absolute number of double-positive T cells is reduced 3- to 4- fold

abnormal CD4-positive, alpha beta T cell morphology ( J:78309 )

• there is reduced surface expression of T cell receptor components compared to mice transgenic for just the TCR transgene

• T cells also express surface markers indicative of recently being activated (high CD44, low CD45RB)

decreased CD4-positive, alpha-beta T cell number ( J:78309 )

• the number of CD4 T cells in the spleen and lymph nodes is severely reduced

• there are 25-fold fewer peripheral T cells present than in Rag2 null homozygotes carrying just the TCR transgene

abnormal CD4-positive, alpha-beta T cell physiology ( J:78309 )

• CD4 T cells have a greatly reduced capability to activate and help B cell antibody production in vivo

abnormal T cell activation ( J:78309 )

• in vitro TCR stimulation leads to greatly reduced expression of CD69 compared to T cells from mice transgenic for just the TCR

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene

cellular

abnormal T cell proliferation ( J:78309 )

• CD4 T cells have a reduced proliferative response to hen egg lysozyme antigen compared to CD4 T cells from mice carrying just the TCR transgene