Phenotypes Associated with This Genotype

Genotype
MGI:3783776

• Allelic Composition: Tg(TcraTcrb)1100Mjb/?
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell proliferation ( J:92867 )

• CD8 single positive T cells of the thymus proliferate vigorously in response to OVA peptide in the context of H2-K^b

abnormal T cell clonal deletion ( J:92867 )

• when OVA peptide is included in fetal thymic organ culture, almost all double-positive T cells are clonally deleted as a result of negative selection

abnormal positive T cell selection ( J:92867 , J:133645 )

• the transgenic T cell receptor (TCR) has high affinity for the H2-K^b allele that leads to increased positive selection of T cell precursors for the CD8 T cell lineage (J:92867)

• the transgenic TCR is positively selected for on this genetic background based on high expression of the TCR on peripheral CD8 T cells (J:133645)

abnormal T cell subpopulation ratio ( J:92867 , J:133645 )

• there is significant skewing in the CD4:CD8 ratio of the thymus towards CD8 single positive T cells (J:92867)

• the ratio of CD4- to CD8- T cells in the periphery is strongly skewed to CD8 T cells (J:133645)

decreased CD4-positive, alpha-beta T cell number ( J:133645 )

• there is approximately a 7-fold reduction in the number of CD4 T cells in the spleen and lymph nodes of 9 week old mice

• there is almost a 5-fold decrease in the percentage of single positive CD4 T cells found in the thymus

abnormal CD8-positive, alpha beta T cell morphology ( J:92867 )

• all CD8 single positive T cells in the thymus express the transgenic TCR that is specific for OVA peptide in the context of H2-K^b

• the CD8 single positive T cells in the thymus express high levels of the transgenic TCR

increased CD8-positive, alpha-beta T cell number ( J:92867 , J:133645 )

• CD8 T cells make up 20% of the total number of cells in the thymus (J:92867)

• there is about a 2-fold increase in the number of CD8 T cells in the spleen and lymph nodes of 9 week old mice (J:133645)

• OVA-specific CD8 T cells make up 50% of the cells found in lymph nodes (J:133645)

• there is over a 6-fold increase in the percentage of single positive CD8 T cells found in the thymus (J:133645)

abnormal cytotoxic T cell physiology ( J:92867 , J:133645 )

• CD8 T cells have high cytolytic activity toward target cells containing OVA peptide (J:92867)

• activated CD8 T cells are strongly cytolytic to cells containing OVA peptide (J:133645)

hematopoietic system

increased T cell proliferation ( J:92867 )

• CD8 single positive T cells of the thymus proliferate vigorously in response to OVA peptide in the context of H2-K^b

abnormal T cell clonal deletion ( J:92867 )

• when OVA peptide is included in fetal thymic organ culture, almost all double-positive T cells are clonally deleted as a result of negative selection

abnormal positive T cell selection ( J:92867 , J:133645 )

• the transgenic T cell receptor (TCR) has high affinity for the H2-K^b allele that leads to increased positive selection of T cell precursors for the CD8 T cell lineage (J:92867)

• the transgenic TCR is positively selected for on this genetic background based on high expression of the TCR on peripheral CD8 T cells (J:133645)

abnormal T cell subpopulation ratio ( J:92867 , J:133645 )

• there is significant skewing in the CD4:CD8 ratio of the thymus towards CD8 single positive T cells (J:92867)

• the ratio of CD4- to CD8- T cells in the periphery is strongly skewed to CD8 T cells (J:133645)

decreased CD4-positive, alpha-beta T cell number ( J:133645 )

• there is approximately a 7-fold reduction in the number of CD4 T cells in the spleen and lymph nodes of 9 week old mice

• there is almost a 5-fold decrease in the percentage of single positive CD4 T cells found in the thymus

abnormal CD8-positive, alpha beta T cell morphology ( J:92867 )

• all CD8 single positive T cells in the thymus express the transgenic TCR that is specific for OVA peptide in the context of H2-K^b

• the CD8 single positive T cells in the thymus express high levels of the transgenic TCR

increased CD8-positive, alpha-beta T cell number ( J:92867 , J:133645 )

• CD8 T cells make up 20% of the total number of cells in the thymus (J:92867)

• there is about a 2-fold increase in the number of CD8 T cells in the spleen and lymph nodes of 9 week old mice (J:133645)

• OVA-specific CD8 T cells make up 50% of the cells found in lymph nodes (J:133645)

• there is over a 6-fold increase in the percentage of single positive CD8 T cells found in the thymus (J:133645)

abnormal cytotoxic T cell physiology ( J:92867 , J:133645 )

• CD8 T cells have high cytolytic activity toward target cells containing OVA peptide (J:92867)

• activated CD8 T cells are strongly cytolytic to cells containing OVA peptide (J:133645)

cellular

increased T cell proliferation ( J:92867 )

• CD8 single positive T cells of the thymus proliferate vigorously in response to OVA peptide in the context of H2-K^b