Phenotypes Associated with This Genotype

Genotype
MGI:3760284

• Allelic Composition: Was^tm1Sbs/Was^tm1Sbs; Wasl^tm2Sbs/Wasl^tm2Sbs; Tg(Lck-cre)1Cwi/?
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

colitis ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells

abnormal double-negative T cell morphology ( J:125309 )

♀ • mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells

♀ • the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Was^tm1Sbs homozygotes)

abnormal double-positive T cell morphology ( J:125309 )

♀ • the percent of double positive (DP) CD69^hi cells is greater than in wild-type

decreased T cell number ( J:125309 )

♀ • the numbers of CD4+ and CD8+ T lymphocytes is reduced

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

decreased regulatory T cell number ( J:125309 )

♀

decreased single-positive T cell number ( J:125309 )

♀ • mice have fewer single positive CD69^hi cells compared to in wild-type mice and Was^tm1Sbs homozygotes

increased single-positive T cell number ( J:125309 )

♀ • the number of single positive CD69^low CD62L^low cells is increased compared to in wild-type mice

hematopoietic system

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells

abnormal double-negative T cell morphology ( J:125309 )

♀ • mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells

♀ • the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Was^tm1Sbs homozygotes)

abnormal double-positive T cell morphology ( J:125309 )

♀ • the percent of double positive (DP) CD69^hi cells is greater than in wild-type

decreased T cell number ( J:125309 )

♀ • the numbers of CD4+ and CD8+ T lymphocytes is reduced

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

decreased regulatory T cell number ( J:125309 )

♀

decreased single-positive T cell number ( J:125309 )

♀ • mice have fewer single positive CD69^hi cells compared to in wild-type mice and Was^tm1Sbs homozygotes

increased single-positive T cell number ( J:125309 )

♀ • the number of single positive CD69^low CD62L^low cells is increased compared to in wild-type mice

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

colitis ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:125309 )

♀ • most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

decreased thymocyte number ( J:125309 )

♀ • the number of thymocytes is reduced

♀ • however, there is no increase in apoptosis

abnormal thymocyte activation ( J:125309 )

♀ • unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation

♀ • spreading of TCR^high CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced

♀ • migratory responses to CCL19 or CXCL12 are reduced even more than in Was^tm1Sbs homozygotes and compared to in wild-type cells