Analysis Tools
immune system
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• mice form germinal centers and have similar numbers of PNAhi, B220+ B cells in spleen
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• following CD40 and Il-4 stimulation, mice have smaller fraction of class-switched cells than control mice (7.4% vs 26.3%); defect appears to be downstream of Cgamma1 germline transcription
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• 14 days after immunization with sheep red blood cells, mutants are observed to lack plasma cells, as recognized by CD138 expression, in spleen, peripheral blood and bone marrow
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• memory B cell generation occurs in mutants after immunization with NP-KLH, but the population is maintained only partly over time; numbers of NP-binding cells are much lower 42 days after immunization compared to controls
• mice receiving a secondary immunization with antigen lack plasmablasts in the spleen that bind NP, are CD138 +ve and have low B220 expression, indicating that memory B cells cannot differentiate into plasma B cells upon antigen restimulation; B cells also lack IgG1 secretion after restimulation
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• mice have much lower titer of IgG1 specific for the hapten NP (nitrophenylacetyl) than controls after immunization with NP coupled to keyhole limpet hemocyanin, but titers of other immunoglobulin classes are similar to levels in controls
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hematopoietic system
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• following CD40 and Il-4 stimulation, mice have smaller fraction of class-switched cells than control mice (7.4% vs 26.3%); defect appears to be downstream of Cgamma1 germline transcription
|
|
• 14 days after immunization with sheep red blood cells, mutants are observed to lack plasma cells, as recognized by CD138 expression, in spleen, peripheral blood and bone marrow
|
|
• memory B cell generation occurs in mutants after immunization with NP-KLH, but the population is maintained only partly over time; numbers of NP-binding cells are much lower 42 days after immunization compared to controls
• mice receiving a secondary immunization with antigen lack plasmablasts in the spleen that bind NP, are CD138 +ve and have low B220 expression, indicating that memory B cells cannot differentiate into plasma B cells upon antigen restimulation; B cells also lack IgG1 secretion after restimulation
|
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• mice have much lower titer of IgG1 specific for the hapten NP (nitrophenylacetyl) than controls after immunization with NP coupled to keyhole limpet hemocyanin, but titers of other immunoglobulin classes are similar to levels in controls
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