Phenotypes Associated with This Genotype

Genotype
MGI:3700771

• Allelic Composition: Amn^amn/Amn^amn
• Genetic Background: involves: 129S1/SvImJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:51542 , J:68615 )

• most die between E9.5 and E10.5, however some continue development to E10.5, with resorption at E11.5 (J:51542)

embryo

embryo phenotype ( J:51542 )

N • Background Sensitivity: amnion is well developed on the mixed 129S1/SvImJ and C57BL/6 background while it is absent on a C57BL/6 background

abnormal ectoderm development ( J:51542 )

• the embryonic ectoderm of E8.5 mutants remains the size of that of a prestreak embryo and appears undifferentiated

• development of the embryonic ectoderm appears delayed by at least 1.5 days

caudal body truncation ( J:51542 )

• homozygotes that survive to E10.5 have a shortened trunk, however they do contain a notochord and a neural tube that has begun to close

embryonic growth arrest ( J:68615 )

absent mesoderm ( J:51542 )

• E8.5 embryos do not express the embryonic mesoderm markers Twist1 and Meox1 (Mox1), indicating that paraxial and lateral mesoderm are either not generated or that their generation is delayed

• homozygotes that survive to E10.5 have barely any mesoderm adjacent to the notochord and neural tube

absent primitive node ( J:51542 )

• a definitive node is absent at E8.5

abnormal primitive streak formation ( J:51542 )

• the middle region of the primitive streak is not appropriately assembled and appears absent

• however, the proximal and distal regions of the streak are properly organized and specified

absent somites ( J:51542 )

• absent somites