Analysis Tools
homeostasis/metabolism
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• platelet thrombus formation is severely impaired ex vivo after treatment of platelets with polyinosinic-polycytidylic acid (PIPC) to induce recombination
• however, initial adhesion of platelets ex vivo is similar to wild-type
• in wild-type mice transfected with Gna13-deficient bone marrow, after vascular injury no thrombi form
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• impairment in shape change and aggregation induced following polyinosinic-polycytidylic acid (PIPC) treatment is identical to that in mice that are also homozygous for Gna12tm1Citb
• serotonin secretion induced by U46619 plus adrenaline or Par-4 peptide exposure is significantly decreased compared to wild-type cells
• however, thrombin-induced serotonin secretion is not impaired
• in wild-type mice transfected with Gna13-deficient bone marrow, platelets adhere to subendothelial surface after injury, but with a ~50% reduction in numbers compared to wild-type platelets
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• very large increase in bleeding time in interferon-responsive tissues
• when bone marrow-derived cells PIPC-treated mice are transplanted into irradiated wild-type mice, 4 weeks later mice have greatly prolonged bleeding times (>20 minutes) compared with mice receiving cells from noninduced controls
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hematopoietic system
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• impairment in shape change and aggregation induced following polyinosinic-polycytidylic acid (PIPC) treatment is identical to that in mice that are also homozygous for Gna12tm1Citb
• serotonin secretion induced by U46619 plus adrenaline or Par-4 peptide exposure is significantly decreased compared to wild-type cells
• however, thrombin-induced serotonin secretion is not impaired
• in wild-type mice transfected with Gna13-deficient bone marrow, platelets adhere to subendothelial surface after injury, but with a ~50% reduction in numbers compared to wild-type platelets
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