Analysis Tools
mortality/aging
|
• lethality between E10.5 and E11.5
|
growth/size/body
|
• slightly smaller at E10.5
|
embryo
|
• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5
• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels
|
|
• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections
|
|
• slightly smaller at E10.5
|
cardiovascular system
|
• show patches of sequestered blood, reflecting dilated vessels, primarily in the upper trunk and head regions
• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries
• para-aortic splanchnopleural mesoderm explants undergo a process in which endothelial cells contract and cluster with each other, resulting in an irregular network composed of wider and smaller vessels
|
|
• isolated endothelial cells grown on fibronectin are poorly spread, lack membrane ruffling and lamellipodia formation and show aberrant locomotion that is faster in the presence of serum and growth supplements
• endothelial cells show fewer and abnormal stress fibers that instead resemble cortical actin bundles and show fewer focal adhesions
|
|
• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries, leading to a great variation in the size of the capillaries and intercapillary spaces
• allantoic explants and para-aortic splanchnopleural mesoderm explants show dilation of capillaries and intercapillary spaces with a reduction in network complexity
|
|
• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression
|
|
• exhibit complete absence of blood vessels in the neuroepithelium at E10.5, indicating defective sprouting angiogenesis into the neuroepithelium
|
|
• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5
• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels
|
|
• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections
|
hemorrhage
(
J:104401
)
|
• hemorrhage is apparent in both the amniotic and yolk sac cavities
|
cellular
|
• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression
|
