Phenotypes for Il5 Il9 MGI:3689874 MGI Mouse

decreased eosinophil cell number ( J:113543 )

• with absence of Il4 and Il5, eosinophilia is completely blocked

abnormal mast cell morphology ( J:113543 )

• mastocytosis is abolished compared to wild-type or triple mutants

abnormal mast cell physiology ( J:113543 )

• no jejunal mast cell protease 1 is produced by mutants compared to wild-type or other allele combinations

abnormal interleukin level ( J:113543 )

• Il-10 expression is reduced compared to Il5^tm1Anjm mutants

abnormal humoral immune response ( J:113543 )

• mice show a switch to a Th1 response and Il12 production from a Th2 response

decreased IgE level ( J:113543 )

• mutants fail to produce IgE, due to deletion of Il4

granulomatous inflammation ( J:113543 )

• primary granuloma formation and volumes in lungs after infection are severely reduced (<10%) compared to wild-type

• secondary granuloma formation is essentially blocked

abnormal susceptibility to infection ( J:113543 )

• expulsion of worms is delayed by an order of magnitude vs triple mutants

increased susceptibility to parasitic infection ( J:113543 )

• compound quadruple mutants show ~1 order of magnitude greater delay in expulsion of worms from intestine (50% remain at day 60 after infection) than triple mutants (35 days)

• more worms remain at 40 days post infection than in triple Il4/Il5/Il13 mutants or wild-type

abnormal intestinal goblet cell morphology ( J:113543 )

• goblet cell hyperplasia is severely impaired after nematode infection with little increase in cell number observed even at 40 days post-infection