Analysis Tools
mortality/aging
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• mutants die at birth
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limbs/digits/tail
short limbs
(
J:114494
)
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• from E14.5 - P0, examination shows that limbs are shorter than in wild-type
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skeleton
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• expansion of Osx1 expression (Osx1-expressing cells) at E14.5, indicating promotion of osteoblast development, is observed
• no Oc+ terminal osteoblasts are found prior to E16.5, and few that are observed are restricted to periosteal region
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• at E16.5, osteoclasts are absent from mutants when they are detected normally in wild-type
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• at E14.5 and 16.5, tibia has abnormal wedge-shaped growth plate with few identifiable hypertrophic chondrocytes visible compared to wild-type
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• at E16.5, no primary spongiosa-like matrix is observed in mutants, unlike wild-type; matrix resembles dense matrix restricted to region forming bone collar
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• at E16.5, long bones appear to have a more intense and broader ossification center than in wild-type
• at P0, a thick bony matrix characterizes all long bones; bone formation is apparent in several cranial regions
• at E14.5, osteoblast lineage is expanded and 3-fold increase in proliferation of osteoblast-forming regions along the length of the periosteum
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• at E14.5, extensive premature bone ossification of the tibia is seen, while no mineralization in wild-type has occurred
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• delayed ossification in skull bones is apparent at E16.5
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hematopoietic system
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• at E16.5, osteoclasts are absent from mutants when they are detected normally in wild-type
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immune system
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• at E16.5, osteoclasts are absent from mutants when they are detected normally in wild-type
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cellular
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• expansion of Osx1 expression (Osx1-expressing cells) at E14.5, indicating promotion of osteoblast development, is observed
• no Oc+ terminal osteoblasts are found prior to E16.5, and few that are observed are restricted to periosteal region
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• at E16.5, osteoclasts are absent from mutants when they are detected normally in wild-type
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