Phenotypes for Cd74 MGI:3689073 MGI Mouse

abnormal B cell differentiation ( J:110927 )

• B cell maturation is defective

increased immature B cell number ( J:110927 )

• higher percentages of immature B cells lacking surface IgD and CD23 expression

decreased CD4-positive, alpha-beta T cell number ( J:110927 , J:113714 )

• mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold (J:110927)

• T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens (J:113714)

abnormal professional antigen presenting cell physiology ( J:113714 )

• splenocytes display enhanced binding capabilities

abnormal dendritic cell antigen presentation ( J:110927 )

• dendritic cells show reduced class II surface expression in overnight culture compared to wild-type

abnormal antigen presentation via MHC class II ( J:110927 , J:113714 )

• mutant spleen cells on an H-2^d background show enhanced peptide-loading capabilities over wild-type cells (J:110927)

• T cell hybridomas produce Il-2 and T cell lines proliferate when stimulated by mutant spleen cells; similar results are seen using T cell clones specific for peptides including ovalbumin (OVA)_323-339 and IgG2a^b (J:110927)

• spleen cells are ineffective for presentation of intact protein antigen as opposed to processed peptide antigens (J:110927)

• spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses (J:110927)

• antigen presentation by mutant spleen cells to E^drestricted T cell clones is substantially decreased (J:113714)

• E^drestricted T cells show enhanced activity when challenged with endogenously process beta 2 microglobulin (B2m) epitopes in presence of mutant splenocytes (J:113714)