Analysis Tools
embryo
| N |
• homozygotes develop normally showing normal histological sections of various organs such as lens, spleen, skeletal and cardiac muscle, lung, kidney, testis, skin, liver, intestine, blood and bone marrow and show normal hair growth and vibrissae development, wound healing, lens differentiation and adipogenesis
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reproductive system
| N |
• exhibit normal reproduction
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cardiovascular system
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• exhibit altered flow-induced arterial remodeling in response to ligation of carotid artery, with occluded carotid arteries displaying less marked reduction of arterial diameter and more pronounced medial hypertrophy than occluded carotid arteries of wild type
• contralateral hyperfused carotid arteries display significant increases in diameter and medial mass, whereas wild-type arteries which experience a more marked increase in blood flow, show no significant structural changes
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cellular
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• fibroblasts 12.5- and 13.5-day old embryos are flatter than wild-type cells which have a spindle-shaped morphology
• mechanical stability of cells in response to distending forces is reduced compared to wild-type cells; mutant fibroblasts exhibit tearing of cytoplasm and rupture of plasma membrane in response to a distending force
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• fibroblasts display reduced contraction of collagenous lattices in collagen gel cultures compared to control cells; contraction forces developed by mutant cells are lower than those observed in wild-type cultures
• actin network formation and focal contact formation are impaired in mutant cells
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• cultured cells show 30% reduction in inherent motility in in vitro wound healing assays, and 40-60% reduction in chemotactic migration induced by PDGF or fibronectin compared to wild-type
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