Phenotypes Associated with This Genotype

Genotype
MGI:3653117

• Allelic Composition: Chd4^tm1.2Kge/Chd4^tm1.2Kge; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes

abnormal T cell differentiation ( J:109547 )

increased double-negative T cell number ( J:109547 )

• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus

decreased double-positive T cell number ( J:109547 )

• percentage of double positive thymocytes is severely reduced

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8+ cells are greatly increased in number

• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

hematopoietic system

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes

abnormal T cell differentiation ( J:109547 )

increased double-negative T cell number ( J:109547 )

• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus

decreased double-positive T cell number ( J:109547 )

• percentage of double positive thymocytes is severely reduced

increased CD8-positive, alpha-beta T cell number ( J:109547 )

• CD8+ cells are greatly increased in number

• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

cellular

abnormal cell cycle ( J:109547 )

• activated T cells are unable to go through normal cell divisions

decreased T cell proliferation ( J:109547 )

• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4^lo and CD8+ CD4- populations

endocrine/exocrine glands

thymus hypoplasia ( J:109547 )

• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes